Rat Skeletal Muscle Nuclei with SYTOX Orange

G-2E/C Bandpass Emission (Narrow Bandwidth Excitation) Green Set

Fluorescence emission intensity from a culture of rat skeletal muscle tissue cells (L6 cell line; myoblasts) that were labeled with SYTOX Orange for nuclear DNA. The visible light absorption maximum of SYTOX Orange is 547 nanometers and the emission maximum occurs at 570 nanometers. In addition, the specimen was simultaneously stained for F-actin with Alexa Fluor 350 conjugated to phalloidin, and for mitochondria with MitoTracker Green FM. Note the presence of substantial signal levels from the red (SYTOX Orange) fluorophore, but the lack of fluorescence intensity from the blue (Alexa Fluor 350) and green (MitoTracker Green FM) probes.