Bovine Pulmonary Artery Cell Nuclei
BFP Bandpass Emission (Narrow Bandwidth Excitation) Violet Set
Fluorescence emission intensity from a culture of bovine pulmonary artery endothelial cells stained with DAPI, which targets DNA in the cell nucleus. The ultraviolet absorption maximum of DAPI (4',6-diamidino-2-phenylindole) is 358 nanometers and the emission maximum occurs at 461 nanometers when the fluorochrome is bound to DNA. In addition, the specimen was simultaneously stained with BODIPY FL phallacidin (targeting actin; green emission) and MitoTracker Red CMXRos (targeting mitochondria; red emission). Note the absence of signal from the red (MitoTracker), and green (BODIPY FL) fluorophores. In addition, the nuclei appear less intense and with fluorescence emission shifted to longer wavelengths than comparable images with the Nikon ultraviolet filter combinations.
