Normal Rabbit Kidney Epithelial Cells (RK-13 Line)
The adherent culture of rabbit kidney epithelial cells (RK-13 line) featured in the image above was transfected with a EYFP-Nucleus subcellular localization vector, thus localizing a green-yellow fluorescent protein tag to the nucleus. After selecting stable transfectants, the cells were treated with MitoTracker Red CMXRos, a synthetic orange-red emitting probe that labels the intracellular mitochondrial network. Images were recorded in grayscale with a 12-bit digital camera coupled to a Nikon TE-2000 inverted microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles. A separate channel was recorded using differential interference contrast and overlaid on the fluorescence channels to identify cell boundaries and common structural features, such as the nucleus.
