The adherent culture of human cervical adenocarcinoma epithelial cells (HeLa line) featured in the image above was transfected with an EGFP-peroxisomes subcellular localization vector, thus localizing a green fluorescent protein tag to the peroxisomal network. Images were recorded in grayscale with a 12-bit digital camera coupled to a Nikon TE-2000 inverted microscope equipped with a bandpass emission fluorescence filter optical block. During the processing stage, the enhanced green fluorescent protein channel was pseudocolored green. A separate channel was recorded using differential interference contrast and overlaid on the fluorescence channel to identify cell boundaries and common structural features, such as the nucleus.