The Nikon MicroscopyU confocal microscopy image gallery was created with a PCM-2000 confocal scanning system interfaced to a Nikon Eclipse E600 upright microscope. Images were recorded in successive z-axis serial sections with C-Imaging Systems software with excitation illumination provided by an argon-ion and/or a helium-neon laser.

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Loose Smut is a fungal disease that replaces developing kernels of wheat grain with black powder-like masses of spores. This pathogenic fungus, referred to by scientists as Ustilago tritici, quietly infects the seeds of cereal crops and stays dormant until spring. Large clouds of black dust arising from combine operations on farms during wheat harvest often reveal smut infestation.

Inside a seedling, parasitic smut sends out fine thread-like filaments called hyphae that feed upon host cells. The creeping filaments form networks that invade almost all plant tissue and form an integrated structure called mycelium. Smut reproduces by forming numerous thick-walled resting spores. Upon maturity, superficial spores erupt through the confines of thin plant membranes and appear as very fine dust-like black powder. The minute black spores travel to other plants upon air currents or are washed into the ground to mix with seed grain during heavy dews or rain.