The body temperature of infants and very young children is often taken through the rectum. This technique is typically employed because rectal temperature is closer to the temperature of the core of the body than oral or other temperature readings. The younger an individual is, the more important such accuracy becomes. Another advantage of rectal temperature is that it is easier to obtain from children too small to cooperate with having their temperature taken orally. Normal rectal human body temperature ranges from 97.6 to 100.4 degrees Fahrenheit.

A rat rectum tissue section (presented above) was stained with Oregon Green 488 conjugated to wheat germ agglutinin in order to target the Golgi apparatus. In addition, the cytoskeletal filamentous actin network was labeled with Alexa Fluor 568 conjugated to phalloidin and cell nuclei were stained with Hoechst 33342. Images were recorded in grayscale with a 12-bit digital camera coupled to a Nikon Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.