The epithelial tissue that lines the seminal vesicles, which are intricately folded organs, is pseudostratified. The secretions of the glands provide nutrients to sperm and help facilitate their movement. Some of the substances contained in the secretions include proteins, fructose, prostaglandins, and enzymes. These materials can sustain sperm for up to three days after ejaculation.

The cytoskeletal filamentous actin network was targeted in a rat seminal vesicle tissue sample with phalloidin conjugated to Alexa Fluor 568. Phalloidin is a member of the phallotoxin group of bicyclic peptides isolated from the deadly Amanita phalloides mushroom. The tissue section was also labeled for nuclear DNA with Hoechst 33342. Images were recorded in grayscale with a 12-bit digital camera coupled to a Nikon Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.