Chinese Hamster Ovary Cells with EGFP and MitoTracker Red CMXRos

FITC-TRITC Bandpass Emission (Dual Band Excitation) Set

Fluorescence emission intensity from a culture of Chinese hamster ovary (CHO line) cells that were transfected with an enhanced green fluorescent protein (EGFP) vector containing complementary DNA to Thy-1, a small glycoprotein residing in the Golgi apparatus. This cell line expresses EGFP-Thy-1 chimeric proteins that are primarily localized to the Golgi, but also distributed into vesicles throughout the cytoplasm. The specimen was labeled with several additional probes, including MitoTracker Red CMXRos (targeting the mitochondria; red fluorescence) and DAPI (targeting the nuclei; blue fluorescence). Note the prominent green staining of the Golgi apparatus in the areas surrounding the nucleus, which is devoid of blue fluorescence from DAPI with this filter combination. Vesicles in the cytoplasm also exhibit green fluorescence, but to a much lesser degree than that seen in the Golgi. In addition, orange-red fluorescence arising from the MitoTracker dye is clearly visible in the mitochondrial network.