Indian Muntjac Cell Actin

B-2A Longpass (Medium Bandwidth Excitation) Blue Set

Fluorescence emission intensity from a culture of Indian Muntjac cells that were labeled with phalloidin conjugated to Alexa Fluor 488 for the intracellular actin cytoskeletal network. The visible light absorption maximum of Alexa Fluor 488 is 495 nanometers and the emission maximum occurs at 519 nanometers. In addition, the specimen was simultaneously labeled with primary anti-human OxPhos Complex V inhibitor protein mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to Alexa Fluor 555 (emission maximum at 571 nanometers). Nuclei were stained with the far-red fluorescent probe TO-PRO-3 (emission maximum at 661 nanometers). Note the presence of moderate signal levels from the orange (Alexa Fluor 555) fluorophore, but the absence of signal from the far-red fluorophore.