Rat Skeletal Muscle Nuclei with SYTOX Green

YFP Bandpass Emission (Narrow Bandwidth Excitation) Blue-Green Set

Fluorescence emission intensity from a culture of rat skeletal muscle tissue cells (L-6 cell line; myoblasts) that were labeled with SYTOX Green for nuclear DNA. In addition, the specimen was simultaneously stained for filamentous actin with the ultraviolet-absorbing probe Alexa Fluor 350 conjugated to phalloidin, and for the intracellular mitochondrial network with MitoTracker Red CMXRos. The visible light absorption maximum of SYTOX Green, when bound to DNA, is 504 nanometers and the emission maximum occurs at 523 nanometers, values that fall within the useful range of the Nikon YFP HYQ filter combination. Note the strong green fluorescence emission from labeled DNA in the cell nuclei, while no signal is observed from the blue and red fluorophores (Alexa Fluor 350 and MitoTracker Red) targeting actin and mitochondria, respectively.