Madin-Darby Bovine Kidney Epithelial Cells (MDBK Line)
The Madin-Darby bovine kidney (MDBK) line was derived from the renal tissue of an adult steer (Bos Taurus) in 1957. The epithelial line was discovered to be infected with bovine viral diarrhea virus (BVDV) in the early 1970s, but in 1982 a subset of the cells was found to be free of the infection, and subsequently this stock came to be the official basis of all future MDBK cells. Thus, modern MDBK cell cultures are BVDV free, though they are susceptible to it and a number of other viruses, including vesicular stomatitis (Indiana strain), infectious bovine rhinotracheitis virus, bovine parvovirus, bovine adenovirus I and III, and parainfluenza virus 3. MDBK cells exhibit resistance to poliovirus 2 and are negative for reverse transcriptase. The epithelial line is utilized in laboratories around the world for a variety of applications, but is perhaps most significant for its use in growing attenuated viruses for vaccine production.