A pair of raccoon uterus fibroblasts distinctly exhibit broad, ruffling lamellipodia along their leading margins. As they migrate across the culture medium, each of the tapering forms is trailed by a long, thin structure known as a retraction fiber. One of the cells can be observed withdrawing its surface extensions in preparation for mitosis and assuming a spherical geometry. Two mononucleated daughter cells are produced, which remain briefly attached to one another, but eventually completely separate as the activity of the surface extensions they form as they settle upon the substratum carry them in opposite directions.
Meanwhile, the retraction fiber of the other PL 1 Ut cell becomes extremely thin as the main process of the cell migrates out of the field of view. When the fiber contracts into a small mound, it becomes apparent that the migrating cell chose to abandon the material, which was likely hindering its advancement across the surface of the imaging chamber. Sometimes large cell fragments will continue dynamic activity for a period of several hours or more after they are produced, but this particular bit of cellular debris seems to promptly cease movement.
Like a scavenger, another PL 1 Ut fibroblast migrates into the otherwise empty field of view and sweeps up the fragment with its lamellipodia. Once it obtains the spoils haphazardly left behind, the fibroblast flees the area. Though it cannot be seen during the video, the material carried along by the cell is likely incorporated into its own cellular contents, as often occurs in culture.