FRET with Spectral Imaging Literature References
Analysis of resonance energy transfer (FRET) between synthetic fluorophores and fluorescent proteins can be readily detected and quantified using spectral imaging and linear unmixing techniques. These probes can be gauged with respect to changes in the emission ratios between the donor and acceptor as a function of added reagents (such as calcium) in fluorescent protein biosensors and with other systems. In addition, spectral imaging can be coupled with acceptor photobleaching FRET techniques to yield quantitative data from spectral profiles obtained before and after bleaching.
Recommended Reading
- Chen, Y., Mauldin, J. P., Day, R. N. and Periasamy, A. Characterization of spectral FRET imaging microscopy for monitoring nuclear protein interactions. Journal of Microscopy 228: 139-152 (2007).
- Dinant, C., Van Royen, M. E., Vermeulen, W. and Houtsmuller, A. B. Fluorescence resonance energy transfer of GFP and YFP by spectral imaging and quantitative acceptor photobleaching. Journal of Microscopy 231: 97-104 (2008).
- Megias, D., Marrero, R., Martinez Del Peso, B., Garcia, M. A., Bravo-Cordero, J. J., Garcia-Grande, A., Santos, A. and Montoya, M. C. Novel lambda FRET spectral confocal microscopy imaging method. Microscopy Research and Technique 72: 1-11 (2009).
- Neher, R. A. and Neher, E. Applying Spectral Fingerprinting to the Analysis of FRET Images. Microscopy Research and Technique 64: 185-195 (2004).
- Thaler, C., Koushik, S. V., Blank, P. S. and Vogel, S. S. Quantitative multiphoton spectral imaging and its use for measuring resonance energy transfer. Biophysical Journal 89: 2736-2749 (2005).
- Zimmermann, T., Rietdorf, J., Girod, A., Georget, V. and Pepperkok, R. Spectral imaging and linear un-mixing enables improved FRET efficiency with a novel GFP2-YFP FRET pair. FEBS Letters 531: 245-249 (2002).
Additional Literature Sources
- Amiri, H., Schultz, G. and Schaefer, M. FRET-based analysis of TRPC subunit stoichiometry. Cell Calcium 33: 463-470 (2003).
- Anderson, K. I., Sanderson, J., Gerwig, S. and Peychl, J. A new configuration of the Zeiss LSM 510 for simultaneous optical separation of green and red fluorescent protein pairs. Cytometry 69A: 920-929 (2006).
- Ecker, R. C., de Rainer, M., Steiner, G. E. and Schmid, J. A. Application of spectral imaging microscopy in cytomics and fluorescence resonance energy transfer (FRET) analysis. Cytometry 59A: 172-181 (2004).
- Goddard, G., Martin, J. C., Naivar, M., Goodwin, P. M., Graves, S. W., Habbersett, R., Nolan, J. P. and Jett, J. H. Single particle high resolution spectral analysis flow cytometry. Cytometry 69A: 842-851 (2006).
- Gu, Y., Di, W. L., Kelsell, D. P. and Zicha, D. Quantitative fluorescence resonance energy transfer (FRET) measurement with acceptor photobleaching and spectral unmixing. Journal of Microscopy 215: 162-173 (2004).
- Hanley, Q. S., Murray, P. I. and Forde, T. S. Microspectroscopic fluorescence analysis with prism-based imaging spectrometers: review and current studies. Cytometry 69A: 759-766 (2006).
- Haraguchi, T., Shimi, T., Koujin, T., Hashiguchi, N. and Hiraoka, Y. Spectral imaging fluorescence microscopy. Genes to Cells 7: 881-887 (2002).
- Hiraoka, Y., Shimi, T. and Haraguchi, T. Multispectral Imaging fluorescence microscopy for living cells. Cell Structure and Function 27: 367-374 (2002).
- Kato, N., Pontier, D. and Lam, E. Spectral profiling for the simultaneous observation of four distinct fluorescent proteins and detection of protein-protein interaction via fluorescence resonance energy transfer in tobacco leaf nuclei. Plant Physiology 129: 931-942 (2002).
- Kho, K. W., Stoddart, P. R., Harris, M. and Mazzolini, A. P. Confocal fluorescence polarization microscopy for linear unmixing of spectrally similar labels. Micron 40: 212-217 (2008).
- McNamara, G., Gupta, A., Reynaert, J., Coates, T. and Boswell, C. Spectral imaging microscopy web sites and data. Cytometry 69A: 863-871 (2006).
- Nadrigny, F., Rivals, I., Hirrlinger, P. G., Koulakoff, A., Personnaz, L., Vernet, M., Allioux, M., Chaumeil, M., Ropert, N., Giaume, C., Kirchoff, F. and Oheim, M. Detecting fluorescent protein expression and co-localisation on single secretory vesicles with linear spectral unmixing. European Biophysics Journal 35: 533-547 (2006).
- Neher, R. A. and Neher, E. Optimizing imaging parameters for the separation of multiple labels in a fluorescence image. Journal of Microscopy 213: 46-62 (2004).
- Ramanjuan, V. K., Biener-Ramanujan, E., Armmer, K., Centonze, V. E. and Herman, B. A. Spectral kinetics ratiometry: a simple approach for real-time monitoring of fluorophores distributions in living cells. Cytometry 69A: 912-919 (2006).
- Spriet, C., Trinel, D., Waharte, F., Deslee, D., Vandenbunder, B., Barbillat, J. and Heliot, L. Correlated fluorescence lifetime and spectral measurements in living cells. Microscopy Research and Technique 70: 85-94 (2007).
- Thaler, C. and Vogel, S. S. Quantitative linear unmixing of CFP and YFP from spectral images acquired with two-photon excitation. Cytometry 69A: 904-911 (2006).
- Zimmermann, T. Spectral imaging and linear unmixing in light microscopy. Advances in Biochemical Engineering and Biotechnology 95: 245-265 (2005).
- Zimmermann, T., Rietdorf, J. and Pepperkok, R. Spectral imaging and its applications in live cell microscopy. FEBS Letters 546: 87-92 (2003).
- Zucker, R., Chua, M., Hooker, M., Salmon, W., Hooker, M., Rigby, P. and Clements, I. Reliability of confocal spectral imaging systems: use of multispectral beads. Cytometry 71A: 174-189 (2007).
