Ground State Depletion Microscopy Literature References
Ground State Depletion (GSD), along with stimulated emission depletion (STED) and saturated structured illumination microscopy (SSIM) are referred to as ensemble focused light imaging techniques, and are based on non-linear optical effects that typically require the application of multiple high-intensity pulsed lasers with specialized modulation filters to control the excitation beam geometry (a technique commonly termed point-spread function engineering). GSD relies on optical shelving, or driving excited state molecules into a dark metastable long-lived triplet state, as a photoswitching mechanism to effectively reduce the point-spread function of the focused excitation laser light.
Recommended Reading
- Bretschneider, S., Eggeling, C. and Hell, S. W. Breaking the diffraction barrier in fluorescence microscopy by optical shelving. Physical Review Letters 98: 21803-4 (2007).
- Folling, J., Bossi, M., Bock, H., Medda, R., Wurm, C. A., Hein, B., Jakobs, S., Eggeling, C. and Hell, S. W. Fluorescence nanoscopy by ground-state depletion and single-molecule return. Nature Methods 5: 943-945 (2008).
- Hackbarth, S. and Roder, B. Anisotropy shape control via light quenching and ground state depletion. Optics Communications 173: 247-254 (2000).
- Hell, S. W. Far-field optical nanoscopy. Science 316: 1153-1158 (2007).
- Hell, S. W., Dyba, M. and Jakobs, S. Concepts for nanoscale resolution in fluorescence microscopy. Current Opinion in Neurobiology 14: 599-609 (2004).
- Hell, S. W. and Kroug, M. Ground-state-depletion fluorescence microscopy: A concept for breaking the diffraction resolution limit. Applied Physics B: Lasers and Optics 60: 495-497 (1995).
- Keller, J., Schonle, A. and Hell, S. W. Efficient fluorescence inhibition patterns for RESOLFT microscopy. Optics Express 15: 3361-3371 (2007).
- Kolmakov, K., Belov, V. N., Bierwagen, J., Ringemann, C., Muller, V., Eggeling, C. and Hell, S. W. Red-emitting rhodamine dyes for fluorescence microscopy and nanoscopy. Chemistry - A European Journal 16: 158-166 (2010).
- Rittweger, E., Wildanger, D. and Hell, S. W. Far-field fluoresence nanoscopy of diamond color centers by ground state depletion. Europhysics Letters 86: 14001-6 (2009).
- van den Engh, G. and Farmer, C. Photo-bleaching and photon saturation in flow cytometry. Cytometry 13: 669-677 (1992).
Additional Literature Sources
- Blom, H., Chmyrov, A., Hassler, K., Davis, L. M. and Widengren, J. Triple-state investigation of fluorescent dyes at dielectric interfaces using total internal reflection fluorescence correlation spectroscopy. Journal of Physical Chemistry A 113: 5554-5566 (2009).
- Burrows, S. M., Reif, R. D., Pappas, D. Investigation of photobleaching and saturation of single molecules by fluorophore recrossing events. Analytica Chimica Acta 598: 135-142 (2007).
- Dittrich, P. S. and Schwille, P. Photobleaching and stabilization of fluorophores used for single-molecule analysis with one- and two-photon excitation. Applied Physics B: Lasers and Optics 73: 829-837 (2001).
- Donnert, G., Eggeling, C. and Hell, S. W. Major signal increase in fluorescence microscopy through dark-state relaxation. Nature Methods 4: 81-86 (2007).
- Han, K. Y., Kim, S. K., Eggeling, C. and Hell, S. W. Metastable dark states enable ground state depletion microscopy of nitrogen vacancy centers in diamond with diffraction-unlimited resolution. Nano Letters 10: 3199-3203 (2010).
- Mondal, P. P. Minimizing photobleaching in fluorescence microscopy by depleting triplet states. Applied Physics Letters 92: 013902-3 (2008).
- Widengren, J., Chmyrov, A., Eggeling, C., Lofdahl, P. and Seidel, C. A. M. Strategies to improve photostabilities in ultrasensitive fluorescence spectroscopy. Journal of Physical Chemistry A 111: 429-440 (2007).
