negative phase contrast microscopy
Phase contrast microscopy where the phase plate retards the un-diffracted light relative to the diffracted light by a quarter wavelength (λ/4). Since diffracted light is retarded by ~λ/4 through interaction with the object, the phase difference is eliminated and constructive interference occurs.
Synonyms: negative phase contrast
See also: phase, phase object, phase plate, phase difference, phase contrast microscopy, interference, positive phase contrast microscopy
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