phase plate

Used in phase contrast microscopy, a phase plate is (usually) placed in the objective lens back focal plane and features a circular modulator conjugate to the circular illumination mask of the phase annulus. It both attenuates light passing through it and advances or retards its phase by a quarter wavelength.

See also: phase annulus , phase contrast microscopy , positive phase contrast microscopy , negative phase contrast microscopy , phase contrast condenser

Learn more:

位相差観察法概論

無色透明の標本に対して高コントラストの像を作成するメカニズム