An increasing number of investigations are using live-cell imaging techniques to provide critical insight into the fundamental nature of cellular and tissue function, especially due to the rapid advances that are currently being witnessed in fluorescent protein and synthetic fluorophore technology.
Förster Resonance Energy Transfer (FRET)
Forster Resonance Energy Transfer occurs between two appropriately positioned fluorophores only when the distance separating them is less than 10 nanometers. Thus, this technique is often used in single molecule assays as well as in intact cells and whole organisms to determine the spatial proximity of molecules.
Fluorescence in situ Hybridization (FISH)
An in situ hybridization approach to labeling DNA and RNA targets in the specimen using oligonucleotide-based fluorescent probes.