phase contrast microscopy

Briefly, a phase annulus is used as a circular illumination mask. A phase plate with matching circular modulator is in a conjugate aperture plane in the detection optics (typically the objective back focal plane). It attenuates and imparts a small phase shift to the un-diffracted 0th order light. Diffracted light (mostly) does not traverse the phase plate and is unattenuated – its interference with 0th order light in the image generates contrast.

Synonyms: phase contrast

See also: phase , phase difference , phase object , phase plate , phase annulus , positive phase contrast microscopy , negative phase contrast microscopy , phase contrast condenser

Learn more:

位相差観察法概論

無色透明の標本に対して高コントラストの像を作成するメカニズム