Normal Human Lung Fibroblast Cells with DAPI, Alexa Fluor 488, and Texas Red

DAPI-FITC-Texas Red Bandpass Emission (Triple Band Excitation) Set

Fluorescence emission intensity from a culture of human lung cells (MRC-5 line) stained with wheat germ agglutinin conjugated to Texas Red, Alexa Fluor 488 conjugated to phalloidin, and DAPI, which target the intracellular Golgi network, cytoskeletal actin filaments, and nucleus, respectively. The visible light absorption maximum of Texas Red is 596 nanometers and the emission maximum occurs at 615 nanometers, while the corresponding values for Alexa Fluor 488 are 495 and 519 nanometers. Note the presence of significant signal levels from all three of the fluorophores employed to stain the specimen, and the high degree of localization for wheat germ agglutinin to the Golgi apparatus.