Light sheet fluorescence microscopy (LSFM) provides an excellent method for 3D and live imaging of large specimens over extended periods of time, utilizing a unique planar illumination approach that minimizes photobleaching and phototoxicity.

Review Articles

Light Sheet Fluorescence Microscopy

Planar illumination techniques for fast 3D imaging of larger specimens with minimal light dosage.

Contributing Author

John R. Allen - Applications and Marketing Specialist, Nikon Instruments Inc., 1300 Walt Whitman Road, Melville NY 11747-3064

Masthead Image Credit

Masthead image recreated under the Creative Commons Attribution License from: Costa A, Candeo A, Fieramonti L, Valentini G, Bassi A (2013) Calcium Dynamics in Root Cells of Arabidopsis thaliana Visualized with Selective Plane Illumination Microscopy. PLoS ONE 8(10): e75646. doi:10.1371/journal.pone.0075646

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