Light sheet fluorescence microscopy (LSFM) provides an excellent method for 3D and live imaging of large specimens over extended periods of time, utilizing a unique planar illumination approach that minimizes photobleaching and phototoxicity.
Planar illumination techniques for fast 3D imaging of larger specimens with minimal light dosage.
John R. Allen - Applications and Marketing Specialist, Nikon Instruments Inc., 1300 Walt Whitman Road, Melville NY 11747-3064
Masthead Image Credit
Masthead image recreated under the Creative Commons Attribution License from: Costa A, Candeo A, Fieramonti L, Valentini G, Bassi A (2013) Calcium Dynamics in Root Cells of Arabidopsis thaliana Visualized with Selective Plane Illumination Microscopy. PLoS ONE 8(10): e75646. doi:10.1371/journal.pone.0075646