Live-Cell Imaging: Cell Motility

Albino Swiss Mouse Embryo
Moloney Murine Leukemia Virus Transfected Cells (CRE BAG 2 Line)

The CRE BAG 2 cell line was established from the NIH 3T3 embryonic Swiss mouse fibroblast cell line, which was transfected with Moloney murine leukemia virus-derived proviral genomes carrying complementary mutations in the gag-pol or env regions. The genomes contained a deletion of the psi sequence needed for the efficient encapsidation of retroviral genomes into virus particles, as well as further alterations at the 3' end of the provirus. CRE BAG2 cells produce a beta-galactosidase-transducing vector (BAG) and are similar to the psi 2 BAG alpha line, though they have apparently never produced a replication-competent helper virus. The fibroblast line can be utilized to package vectors derived from murine leukemia viruses and is positive for reverse transcriptase.

CRE BAG 2 Live Cell Video No. 1 - Several CRE BAG 2 fibroblasts carry out the process of endocytosis, vacuoles forming along their peripheries and appearing to stream into the central regions of the cells. Choose a playback format that matches your connection speed: 56.6k (modem), or T1/Cable/DSL (RealPlayer), or T1/Cable/DSL (Windows Media), or download this video clip in MPEG format (22.0 hour time-lapse sequence; 66 seconds - 27.6 MB).

CRE BAG 2 Live Cell Video No. 2 - Cultured CRE BAG 2 cells move about the culture environment via repeated cycles of lamellipodia extension and contraction. Choose a playback format that matches your connection speed: 56.6k (modem), or T1/Cable/DSL (RealPlayer), or T1/Cable/DSL (Windows Media), or download this video clip in MPEG format (28.6 hour time-lapse sequence; 86 seconds - 36.1 MB).

CRE BAG 2 Live Cell Video No. 3 - CRE BAG 2 fibroblasts that come into contact with cell fragments often attempt to incorporate the material into their own cellular contents. Choose a playback format that matches your connection speed: 56.6k (modem), or T1/Cable/DSL (RealPlayer), or T1/Cable/DSL (Windows Media), or download this video clip in MPEG format (18.6 hour time-lapse sequence; 56 seconds - 23.7 MB).

CRE BAG 2 Live Cell Video No. 4 - The long, adherent retraction fibers of many of the CRE BAG 2 fibroblasts seem to intertwine like the threads of a spider’s web. Choose a playback format that matches your connection speed: 56.6k (modem), or T1/Cable/DSL (RealPlayer), or T1/Cable/DSL (Windows Media), or download this video clip in MPEG format (20.6 hour time-lapse sequence; 62 seconds - 26.2 MB).