Live-Cell Imaging: Cell Motility

Raccoon Uterus Fibroblast Cells (PL 1 Ut Line)

The PL 1 Ut cell line was derived from the uterine tissue of an adult female North American raccoon (Procyon lotor). The cells exhibit fibroblast-like morphological characteristics and grow adherently to glass and polymer surfaces in culture. Studies have shown that PL 1 Ut cells are susceptible to an array of viruses, including herpes simplex virus, reovirus 3, and vesicular stomatitis (Ogden strain). The line is commonly utilized in the propagation of such viruses for research purposes, and has been particularly useful in investigations of feline and canine viral diseases. PL 1 Ut cells are resistant to infection with poliovirus 2, coxsackieviruses A9 and B5, vaccinia, and adenoviruses 2 and 5.

PL 1 Ut Live Cell Video No. 1 - Like a scavenger, a PL 1 Ut fibroblast migrates into an otherwise empty field of view and sweeps up a fragment left haphazardly behind by another cell with its lamellipodia. Choose a playback format that matches your connection speed: 56.6k (modem), or T1/Cable/DSL (RealPlayer), or T1/Cable/DSL (Windows Media), or download this video clip in MPEG format (16.6 hour time-lapse sequence; 50 seconds - 21.2 MB).

PL 1 Ut Live Cell Video No. 2 - Located inside of the nuclei of the raccoon uterus fibroblasts are nucleoli, which are small, dense bodies that serve as sites of ribosomal RNA synthesis. Choose a playback format that matches your connection speed: 56.6k (modem), or T1/Cable/DSL (RealPlayer), or T1/Cable/DSL (Windows Media), or download this video clip in MPEG format (19.6 hour time-lapse sequence; 59 seconds - 24.8 MB).

PL 1 Ut Live Cell Video No. 3 - A cleavage furrow is formed and cytokinesis is completed, producing a pair of daughter cells that develop their own polarities and begin migrating in disparate directions. Choose a playback format that matches your connection speed: 56.6k (modem), or T1/Cable/DSL (RealPlayer), or T1/Cable/DSL (Windows Media), or download this video clip in MPEG format (21.0 hour time-lapse sequence; 63 seconds - 26.5 MB).

PL 1 Ut Live Cell Video No. 4 - PL 1 Ut cell fragments are produced when focal adhesions between a fibroblast and the substratum are not properly released, forcing the cell to take the draconian measure of severing pieces of its own cytoplasmic material in order to continue unhindered along its path. Choose a playback format that matches your connection speed: 56.6k (modem), or T1/Cable/DSL (RealPlayer), or T1/Cable/DSL (Windows Media), or download this video clip in MPEG format (23.3 hour time-lapse sequence; 70 seconds - 29.4 MB).

PL 1 Ut Live Cell Video No. 5 - The leading margins of migrating PL 1 Ut fibroblasts are generally broad, being comprised of flattened sheets of membrane-enclosed cytoplasm called lamellipodia. Choose a playback format that matches your connection speed: 56.6k (modem), or T1/Cable/DSL (RealPlayer), or T1/Cable/DSL (Windows Media), or download this video clip in MPEG format (15.3 hour time-lapse sequence; 46 seconds - 19.5 MB).