Check out this new open-access resource article from researchers at Yale University detailing a 4Pi single molecule localization microscope with isotropic localization precision of ~10-20 nm. Importantly this new nanoscope can be used to image samples up to 10 um thick, a significant improvement over previous two-objective localization microscopy approaches. This is made possible by using deformable mirrors to correct for depth-induced changes in the shape of the point-spread function.