Bimolecular Fluorescence Complementation Literature References
Bimolecular fluorescence complementation (BiFC) is a technique that enables direct visualization of protein interactions in living cells. The methodology is based on the association between two non-fluorescent fragments of a fluorescent protein that begin to emit fluorescence when brought into close proximity by fusion partners that are capable of interacting. A number of protein interactions have been investigated using BiFC in many different cell types and organisms.
Recommended Reading
- Kerppola, T. K. Bimolecular fluorescence complementation (BiFC) analysis as a probe of protein interactions in living cells. Annual Review of Biophysics 37: 465-487 (2008).
- Kerppola, T. K. Visualization of molecular interactions by fluorescence complementation. Nature Reviews Molecular Cell Biology 7: 449-456 (2006).
- Lalonde, S., Ehrhardt, D. W. and Frommer, W. B. Shining light on signaling and metabolic networks by genetically encoded biosensors. Current Opinion in Plant Biology 8: 574-581 (2005).
- Cabantous, S., Terwilliger, T. C. and Waldo, G. S. Protein tagging and detection with engineered self-assembling fragments of green fluorescent protein. Nature Biotechnology 23: 102-107 (2005).
- Bracha-Drori, K., Shichrur, K., Katz, A., Oliva, M., Angleovic, R., Yalovsku, S. and Ohad, N. Detection of protein-protein interactions in plants using bimolecular fluorescence complementation. The Plant Journal 40: 419-427 (2004).
- Hu, C. D. and Kerppola, T. K. Simultaneous visualization of multiple protein interactions in living cells using multicolor fluorescence complementation analysis. Nature Biotechnology 21: 539-545 (2003).
- Fan, J., Cui, Z., Wei, H., Zhang, Z., Zhou, Y., Wang, Y. and Zhang, X. Split mCherry as a new red bimolecular fluorescence complementation system for visualizing protein-protein interactions in living cells. Biochemical and Biophysical Research Communications 367: 47-53 (2008).
Additional Literature Sources
- Barnard, E., McFerran, N. V., Trudgett, A., Nelson, J. and Timson, D. J. Development and implementation of split-GFP-based bimolecular fluorescence complementation (BiFC) assays in yeast. Biochemical Society Transactions 36: 479-482 (2008).
- Bhat, R. A., Lahaye, T. and Panstruga, R. The visible touch: In planta visualization of protein-protein interactions by fluorophore-based methods. Plant Methods 2: 1-14 (2006).
- Cabantous, S. and Waldo, G. S. In vivo and in vitro protein solubility assays using split GFP. Nature Methods 3: 845-854 (2006).
- Cabantous, S., Pedelacq, J., Mark, B. L., Naranjo, C., Terwilliger, T. C. and Waldo, G. S. Recent advances in GFP folding reported and split-GFP solubility reporter technologies. Application to improving the folding and solubility of recalcitrant proteins from Mycobacterium tuberculosis. Journal of Structural and Functional Genomics 6: 113-119 (2005).
- Citovsky, V., Lee, L., Vyas, S., Glick, E., Chen, M., Vainstein, A., Gafni, Y., Gelvin, S. B. and Tzfira, T. Subcellular localization of interacting proteins by bimolecular fluorescence complementation in Planta. Journal of Molecular Biology 362: 1120-1131 (2006).
- Cole, K. C., McLaughlin, H. W. and Johnson, D. I. Use of bimolecular fluorescence complementation to study in vivo interactions between Cdc42p and Rdi1p of Saccharomyces cerevisiae. Eukaryotic Cell 6: 378-387 (2007).
- de Virgilio, M., Kiosses, W. B. and Shattil, S. J. Proximal, selective, and dynamic interactions between integrin allbb3 and protein tyrosine kinases in living cells. The Journal of Cell Biology 165: 305-311 (2004).
- Demidov, V. V. and Broude, N. E. Profluorescent protein fragments for fast bimolecular fluorescence complementation in vitro. Nature Protocols 1: 714-719 (2006).
- Demidov, V. V., Dokholyan, N. V., Witte-Hoffmann, C., Chalasani, P., Yiu, H., Ding, F., Yu, Y., Cantor, C. R. and Broude, N. E. Fast complementation of split fluorescent protein triggered by DNA hybridization. Proceedings of the National Academy of Sciences (USA) 103: 2052-2056 (2006).
- Ghosh, I., Hamilton, A. D. and Regan, L. Antiparallel leucine zipper-directed protein reassembly: Application to the green fluorescent protein. Journal of the American Chemical Society 122: 5658-5659 (2000).
- Grinberg, A. V., Hu, C. and Kerppola, T. K. Visualization of Myc/Max/Mad family dimmers and the competition for dimerization in living cells. Molecular and Cellular Biology 24: 4294-4308 (2004).
- Haim, L., Zipor, G., Aronov, S. and Gerst, J. E. A genomic integration method to visualize localization of endogenous mRNAs in living yeast. Nature Methods 4: 409-412 ().
- Hu, C., Chinenov, Y. and Kerppola, T. K. Visualization of interactions among bZIP and Rel family proteins in living cells using bimolecular fluorescence complementation. Molecular Cell 9: 789-798 (2002).
- Jach, G., Pesch, M., Richter, K., Frings, S. and Uhrig, J. F. An improved mRFP1 adds red to bimolecular fluorescence complementation. Nature Methods 3: 597-600 (2006).
- Jeong, J., Kim, S. K., Ahn, J., Park, K., Jeong, E., Kim, M. and Chung, B. H. Monitoring of conformational change in maltose binding protein using split green fluorescent protein. Biochemical and Biophysical Research Communications 339: 647-651 (2006).
- Kaihara, A., Kawai, Y., Sato, M., Ozawa, T. and Umezawa, Y. Locating a protein-protein interaction in living cells via split Renilla luciferase complementation. Analytical Chemistry 75: 4176-4181 (2003).
- Kim, S. B., Ozawa, T., Watanabe, S. and Umezawa, Y. High-throughput sensing and noninvasive imaging of protein nuclear transport by using reconstitution of split Renilla luciferase. Proceedings of the National Academy of Sciences (USA) 101: 11542-11547 (2004).
- Magliery, T. J., Wilson, C. G. M., Pan, W., Mishler, D., Ghosh, I., Hamilton, A. D. and Regan, L. Detecting protein- protein interactions with a green fluorescent protein fragment reassembly trap: Scope and mechanism. Journal of the American Chemical Society 127: 146-157 (2005).
- Michnick, S. W., Ear, P. H., Manderson, E. N., Remy, I. and Stefan, E. Universal strategies in research and drug discovery based on protein-fragment complementation assays. Nature Reviews Drug Discovery 6: 570-582 (2007).
- Ozawa, T. and Umezawa, Y. Detection of protein-protein interaction in vivo based on protein slicing. Current Opinion in Chemical Biology 5: 578-583 (2001).
- Ozawa, T. and Umezawa, Y. Peptide assemblies in living cells. Methods for detecting protein-protein interactions. Supramolecular Chemistry 14: 271-280 (2002).
- Ozawa, T., Natori, Y., Sato, M. and Umezawa, Y. Imaging dynamics of endogenous mitochondrial RNA in single living cells. Nature Methods 4: 413-419 (2007).
- Ozawa, T., Nogami, S., Sato, M., Ohya, Y. and Umezawa, Y. A fluorescent indicator for detecting protein-protein interactions in vivo based on protein splicing. Analytical Chemistry 72: 5151-5157 (2000).
- Ozawa, T., Kaihara, A., Moritoshi, S., Tachihara, K. and Umezawa, Y. Split luciferase as an optical probe for detecting protein-protein interactions in mammalian cells based on protein splicing. Analytical Chemistry 73: 2516-2521 (2001).
- Ozawa, T., Takeuchi, M., Kaihara, A., Sato, M. and Umezawa, Y. Protein splicing-based reconstitution of split green fluorescent protein for monitoring protein-protein interactions in bacteria: Improved sensitivity and reduced screening time. Analytical Chemistry 73: 5866-5874 (2001).
- Ozawa, T., Sako, Y., Sato, M., Kitamura, T. and Umezawa, Y. A genetic approach to identifying mitochondrial proteins. Nature Biotechnology 21: 287-293 (2003).
- Remy, I. and Michnick, S. W. A cDNA library functional screening strategy based on fluorescent protein complementation assays to identify novel components of signaling pathways. Methods 32: 381-388 (2004).
- Remy, I. and Michnick, S. W. A highly sensitive protein-protein interaction assay based on Gaussia luciferase. Nature Methods 3: 977-979 (2006).
- Stains, C. I., Porter, J. R., Ooi, A. T., Segal, D. J. and Ghosh, I. DNA sequence-enabled reassembly of the green fluorescent protein. Journal of the American Chemical Society 127: 10782-10783 (2005).
- Stefan, E., Aquin, S., Berger, N., Landry, C. R., Nyfeler, B., Bouvier, M. and Michnick, S. W. Quantification of dynamic protein complexes using Renilla luciferase fragment complementation applied to protein kinase A activities in vivo. Proceedings of the National Academy of Sciences (USA) 104: 16916-16921 (2007).
- Stolpe, T., SuBlin, C., Marrocco, K., Nick, P., Kretsch, T. and Kircher, S. In planta analysis of protein-protein interactions related to light signaling by bimolecular fluorescence complementation. Protoplasma 226: 137-146 (2005).
- Torrado, M., Iglesias, R. and Mikhailov, A. T. Detection of protein interactions based on GFP fragment complementation by fluorescence microscopy and spectrofluorometry. BioTechniques 44: 70-74 (2008).
- Tyagi, S. Splitting or stacking fluorescent proteins to visualize mRNA in living cells. Nature Methods 4: 391-392 (2007).
- Valencia-Burton, M., McCullough, R. M., Cantor, C. R. and Broude, N. E. RNA visualization in live bacterial cells using fluorescent protein complementation. Nature Methods 4: 421-427 (2007).
- Walter, M., Chaban, C., Schutze, K., Batistic, O., Weckermann, K., Nake, C., Blazevic, D., Grefen, C., Schumacher, K., Oecking, C., Harter, K. and Kudla, J. Visualization of protein interactions in living plant cells using bimolecular fluorescence complementation. The Plant Journal 40: 428-438 (2004).
- Wilson, C. G. M., Magliery, T. J. and Regan, L. Detecting protein-protein interactions with GFP-fragment reassembly. Nature Methods 1: 255-262 (2004).
- Zhang, S., Ma, C. and Chalfie, M. Combinatorial marking of cells and organelles with reconstituted fluorescent proteins. Cell 119: 137-144 (2004).
