dStorm Superresolution Literature References
The technique of direct stochastic optical reconstruction microscopy (dSTORM) differs from the parent technique by employing conventional carbocyanine dyes for cellular staining and does not rely on the proximity of two fluorophores attached to the same targeting antibody in a specific ratio. The original research report on dSTORM used Cy5 and Alexa Fluor 647 to demonstrate the novel single-molecule superresolution technique, which requires substantial laser power to ensure effective transition of the photoswitchable fluorophores into a dark state.
Recommended Literature
- Baddeley, D., Jayasinghe, I. D., Cremer, C., Cannell, M. B. and Soeller, C. Light-induced dark states of organic fluochromes enable 30 nm resolution imaging in standard media.Biophysical Journal 96: L22-L32 (2009).
- Bossi, M., Folling, J., Belov, V. N., Boyarskiy, V. P., Medda, R., Egner, A., Eggeling, C., Schonle, A. and Hell, S. W. Multicolor far-field fluorescence nanoscopy through isolated detection of distinct molecular species. Nano Letters 8: 2463-2468 (2008).
- Cordes, T., Stein, I. H., Forthmann, C., Steinhauer, C., Walz, M., Summerer, W., Person, B., Vogelsang, J. and Tinnefeld, P. Controlling the emission of organic dyes for high sensitivity and super-resolution microscopy. Proceedings of SPIE 131: 73671D-2 (2009).
- Folling, J., Bossi, M., Bock, H., Medda, R., Wurm, C. A., Hein, B., Jakobs, S., Eggeling, C. and Hell, S. W. Fluorescence nanoscopy by ground-state depletion and single-molecule return.Nature Methods 5: 943-945 (2008).
- Heilemann, M., van de Linde, S., Mukherjee, A. and Sauer, M. Super-resolution imaging with small organic fluorophores. Angewandte Chemie International Edition 48: 6903-6908 (2009).
- Heilemann, M., van de Linde, S., Schuttpelz, M., Kasper, R., Seefeldt, B., Mukherjee, A., Tinnefeld, P. and Sauer, M. Subdiffraction-resolution fluorescence imaging with conventional fluorescent probes. Angewandte Chemie International Edition 47: 6172-6176 (2008).
- Schuttpelz, M., Wolter, S., van de Linde, S., Heilemann, M. and Sauer, M. dSTORM: Real-time subdiffraction-resolution fluorescence imaging with organic fluorophores. Proceedings of SPIE 7571: 75710V-2(2010).
- Wolter, S., Schuttpelz, M., Tscherepanow, M., van de Linde, S., Heilemann, M. and Sauer, M. Real-time computation of subdiffraction-resolution fluorescence images. Journal of Microscopy 237: 12-22 (2010).
Additional Literature Sources
- Endesfelder, U., van de Linde, S., Wolter, S., Sauer, M. and Heilemann, M. Subdiffraction-resolution fluorescence microscopy of myosin-actin motility. ChemPhysChem 11: 836-840 (2010).
- Gunkel, M., Erdel, F., Rippe, K., Lemmer, P., Kaufmann, R., Hormann, C., Amberger, R. and Cremer, C. Dual color localization microscopy of cellular nanostructures. Biotechnology Journal 4: 927-938 (2009).
- Lemmer, P., Gunkel, M., Weiland, Y., Muller, P., Baddeley, D., Kaufmann, R., Urich, A., Eipel, H., Amberger, R., Hausmann, M. and Cremer, C. Using conventional fluorescent markers for far-field fluorescence localization nanoscopy allows resolution in the 10-nm range. Journal of Microscopy 235: 163-171 (2009).
- Rundquist, I. and Enerback, L. Millisecond fading and recovery phenomena in fluorescent biological objects. Histochemistry (and Cell Biology) 47: 79-87 (1975).
- Steinhauer, C., Forthmann, C., Vogelsang, J. and Tinnefeld, P. Superresolution microscopy on the basis of engineered dark states. Journal of the American Chemical Society 130: 16840-16841 (2008).
- Steinhauer, C., Jungmann, R., Sobey, T. L., Simmel, F. C. and Tinnefeld, P. DNA origami as a nanoscopic ruler for super-resolution microscopy. Angewandte Chemie International Edition 48: 8870-8873 (2009).
- van de Linde, S., Kasper, R., Heilemann, M. and Sauer, M. Photoswitching microscopy with standard fluorophores. Applied Physics B: Lasers and Optics 93: 725-731 (2008).
- van de Linde, S., Sauer, M. and Heilemann, M. Subdiffraction-resolution fluorescence imaging of proteins in the mitochondrial inner membrane with photoswitchable fluorophores. Journal of Structural Biology 164: 250-254 (2008).
- van de Linde, S., Wolter, S., Heilemann, M. and Sauer, M. The effect of photoswitching kinetics and labeling densities on super-resolution fluorescence imaging. Journal of Biotechnology 149: 260-266 (2010).
- Vogelsang, J., Cordes, T., Forthmann, C., Steinhauer, C. and Tinnefeld, P. Controlling the fluorescence of ordinary oxazine dyes for single-molecule switching and superresolution microscopy. Proceedings of the National Academy of Sciences (USA) 106: 8107-8112 (2009).
- Vogelsang, J., Kasper, R., Steinhauer, C., Person, B., Heilemann, M. Sauer, M. and Tinnefeld, P. A reducing and oxidizing system minimizes photobleaching and blinking of fluorescent dyes. Angewandte Chemie International Edition 47: 5465-5469 (2008).
- Westphal, V. and Hell, S. W. Nanoscale resolution in the focal plane of an optical microscope. Physical Review Letters 94: 143903-4 (2005).
- Zondervan, R., Kulzer, F., Orlinskii, S. B. and Orrit, M. Photoblinking of rhodamine 6G in poly(vinyl alcohol): Radical dark state formed through the triplet. Journal of Physical Chemistry A 107: 6770-6776 (2003).
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