African Green Monkey Kidney Cell Mitochondria
G-2B Longpass Emission (Wide Bandwidth Excitation) Green Set
Fluorescence emission intensity from a culture of African green monkey (COS-1 line) cells stained with MitoTracker Red CMXRos, which targets the intracellular mitochondrial network. The visible light absorption maximum of MitoTracker Red CMXRos is 579 nanometers and the emission maximum occurs at 599 nanometers. In addition, the specimen was simultaneously stained with DAPI (targeting DNA in the cell nucleus; blue emission) and Alexa Fluor 488 conjugated to phalloidin (targeting actin; green emission). Note the absence of signal from the blue DAPI and green fluorophores, but the bright orange-red fluorescence exhibited by the disc-shaped mitochondria.