African Green Monkey Kidney Cells with QDot 525
YFP Bandpass Emission (Narrow Bandwidth Excitation) Blue-Green Set
Fluorescence emission intensity from a culture of African green monkey (COS-1 line) cells that were immunofluorescently labeled with primary anti-cytokeratin mouse monoclonal antibodies followed by goat anti-mouse F(ab')2 fragments conjugated to biotin. The secondary antibody was subsequently stained with quantum dots (QDot 525) conjugated to streptavidin. In addition, the specimen was simultaneously stained for DNA with the ultraviolet-absorbing probe DAPI, and for the intracellular mitochondrial network with MitoTracker Red CMXRos. In COS-1 cells, the keratin protein network is not as organized and clearly defined as is observed with other cell types. QDot 525 conjugates exhibit absorption over a broad wavelength range extending from the ultraviolet to near their maximum emission wavelength of approximately 525 nanometers, values that fall within the useful range of the Nikon YFP HYQ filter combination. Note the high level of green fluorescence observed from the keratin intermediate filament network, but an absence of signal from the blue and red fluorophores (DAPI and MitoTracker) targeting the nuclei and mitochondria, respectively.