Albino Swiss Mouse Embryo Cell Tubulin with Cy2
YFP Bandpass Emission (Narrow Bandwidth Excitation) Blue-Green Set
Fluorescence emission intensity from a culture of albino Swiss mouse embryo cells (3T3line) that were immunofluorescently labeled with primary anti-bovine alpha-tubulin mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to Cy2. In addition, the specimen was simultaneously stained for DNA with the ultraviolet-absorbing probe DAPI, and for the cytoskeletal filamentous actin network with Alexa Fluor 568 conjugated to phalloidin. The visible light absorption maximum of Cy2 is 489 nanometers and the emission maximum occurs at 506 nanometers, values that fall within the useful range of the Nikon YFP HYQ filter combination. Note the high level of green fluorescence observed from the intracellular microtubule network that extends throughout the cytoplasm, but an absence of signal from the blue and orange fluorophores (DAPI and Alexa Fluor 568) targeting the nuclei and actin, respectively.