Albino Swiss Mouse Embryo Moloney Murine Leukemia Virus Transfected Cells (CRE BAG 2 Line)
The CRE BAG 2 cell line was established from the NIH 3T3 embryonic Swiss mouse fibroblast cell line, which was transfected with Moloney murine leukemia virus-derived proviral genomes carrying complementary mutations in the gag-pol or env regions. The genomes contained a deletion of the psi sequence needed for the efficient encapsidation of retroviral genomes into virus particles, as well as further alterations at the 3' end of the provirus. CRE BAG2 cells produce a beta-galactosidase-transducing vector (BAG) and are similar to the psi 2 BAG alpha line, though they have apparently never produced a replication-competent helper virus. The fibroblast line can be utilized to package vectors derived from murine leukemia viruses and is positive for reverse transcriptase.