Bovine Pulmonary Artery Cell Actin
B-1A Longpass Emission (Narrow Bandwidth Excitation) Blue Set
Fluorescence emission intensity from a culture of bovine pulmonary artery endothelial cells stained with BODIPY FL phallacidin, which binds to the intracellular filamentous actin network. The visible light absorption maximum of BODIPY FL is 503 nanometers and the emission maximum occurs at 512 nanometers. In addition, the specimen was simultaneously stained with DAPI (targeting DNA in the cell nucleus; blue emission) and MitoTracker Red CMXRos (targeting mitochondria; red emission). Note the presence of signal from the red fluorophore (MitoTracker), but the absence of blue fluorescence from DAPI, which is not efficiently excited in this spectral region.