Bovine Pulmonary Artery Cell Actin and Tubulin
FITC-TRITC Bandpass Emission (Dual Band Excitation) Set
Fluorescence emission intensity from a culture of bovine pulmonary artery endothelial cells that were immunofluorescently labeled with primary anti-bovine alpha-tubulin mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to BODIPY FL. The cell culture was simultaneously stained with Texas Red phalloidin and DAPI, which target the cytoskeletal actin network and DNA in the nucleus, respectively. The visible light absorption maximum of BODIPY FL is 505 nanometers and the emission maximum occurs at 513 nanometers, while the corresponding values for Texas Red are 595 and 620 nanometers. Note the absence of signal from the blue (DAPI) fluorophore, but the bright red fluorescence exhibited by the actin filaments in the cytoplasm and the green emission from the microtubule network.