Bovine Pulmonary Artery Cell Nuclei

BV-2B Longpass Emission (Medium Bandwidth Excitation) Blue-Violet Set

Fluorescence emission intensity from a culture of bovine pulmonary artery endothelial cells stained with DAPI, which targets DNA in the cell nucleus. The ultraviolet absorption maximum of DAPI (4',6-diamidino-2-phenylindole) is 358 nanometers and the emission maximum occurs at 461 nanometers when the fluorochrome is bound to DNA. The specimen was also simultaneously stained with BODIPY FL phallacidin (targeting actin; green emission) and MitoTracker Red CMXRos (targeting mitochondria; red emission). Note the presence of substantial signal from the green (BODIPY FL) probe, but lower signal levels from the red (MitoTracker) and blue (DAPI) probes. In addition, the nuclei appear far less intense and with fluorescence emission shifted to longer wavelengths than comparable images with the Nikon violet and ultraviolet filter combinations.

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