Bovine Pulmonary Artery Cell Nuclei

CFP Bandpass Emission (Narrow Bandwidth Excitation) Blue-Violet Set

Fluorescence emission intensity from a culture of bovine pulmonary artery endothelial cells stained with DAPI, which targets DNA in the cell nucleus. The ultraviolet absorption maximum of DAPI (4',6-diamidino-2-phenylindole) is 358 nanometers and the emission maximum occurs at 461 nanometers when the fluorochrome is bound to DNA. In addition, the specimen was simultaneously stained with BODIPY FL phallacidin (targeting actin; green emission) and MitoTracker Red CMXRos (targeting mitochondria; red emission). Note the absence of signal from the red (MitoTracker) fluorophore, but bleed-through from the green (BODIPY FL) probe, which appears as a weak cyan signal surrounding the nuclei. In addition, the nuclei appear less intense and with fluorescence emission shifted to longer wavelengths than comparable images with the Nikon violet and ultraviolet filter combinations.

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