Bovine Pulmonary Artery Cell Nuclei

UV-2B Longpass Emission (Wide Bandwidth Excitation) Ultraviolet Set

Fluorescence emission intensity from a culture of bovine pulmonary artery endothelial cells stained with DAPI, which targets DNA in the cell nucleus. The ultraviolet absorption maximum of DAPI (4',6-diamidino-2-phenylindole) is 358 nanometers and the emission maximum occurs at 461 nanometers when the fluorochrome is bound to DNA. In addition, the specimen was simultaneously stained with BODIPY FL phallacidin (targeting actin; green emission) and MitoTracker Red CMXRos (targeting mitochondria; red emission). Note the absence of signal from the red fluorophore (MitoTracker), but the presence of green fluorescence from the actin cytoskeletal network. In addition, the nuclei appear less intense and with fluorescence emission shifted to higher wavelengths than comparable images with the Nikon UV-2E/C bandpass filter set. However, the image has higher contrast and a darker background than similar specimens observed with the Nikon longpass UV-2A filter combination.

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