Bovine Pulmonary Artery Keratin Network
G-1A Longpass Emission (Narrow Bandwidth Excitation) Green Set
Fluorescence emission intensity from a culture of bovine pulmonary artery endothelial cells that were immunofluorescently labeled with primary anti-cytokeratin mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to Alexa Fluor 546. The visible light absorption maximum of Alexa Fluor 546 is 556 nanometers and the emission maximum occurs at 573 nanometers (in the yellow region of the visible light spectrum). Keratins are a class of water-insoluble proteins that polymerize to form monofilaments, a class of intermediate filaments that forms part of the cytoskeletal complex in many epithelial tissues. Because the composition of keratin filaments varies widely with species and cell type, the microfilament network is not always clearly delineated when labeled with a specific antibody, as occurs in the specimen presented above.