Canine Kidney Cell Mitochondria with Fluorescein
YFP Bandpass Emission (Narrow Bandwidth Excitation) Blue-Green Set
Fluorescence emission intensity from a culture of canine kidney epithelial cells (Madin-Darby; MDCK line) that were immunofluorescently labeled with primary anti-oxphos complex V inhibitor protein monoclonal antibodies (mouse) followed by goat anti-mouse Fabfragments conjugated to fluorescein (FITC). In addition, the specimen was simultaneously stained for DNA with the ultraviolet-absorbing probe DAPI, and for the cytoskeletal filamentous actin network with Alexa Fluor 568 conjugated to phalloidin. The visible light absorption maximum of fluorescein is 490 nanometers and the emission maximum occurs at 525 nanometers, values that fall within the useful range of the Nikon YFP HYQ filter combination. Note the high level of green fluorescence from the labeled intracellular mitochondria, but an absence of detected emission from the blue and orange fluorophores (DAPI and Alexa Fluor 568) targeting the nuclei and actin, respectively.