Canine Kidney Epithelial Cellular Cytoskeletal F-Actin Network with Alexa Fluor 633

Cy5 HYQ Bandpass Emission (Wide Bandwidth Excitation) Red Set

Fluorescence emission intensity from a culture of canine kidney epithelial (Madin-Darby;MDCK line) cells stained with phalloidin conjugated to Alexa Fluor 633, which targets the intracellular cytoskeletal F-actin network. Phalloidins are a member of the phallotoxin group of bicyclic peptides isolated from the deadly Amanita phalloides mushroom. The visible light absorption maximum of Alexa Fluor 633 is 621 nanometers and the emission maximum occurs at 639 nanometers. The image was captured in 12-bit grayscale mode, but subsequently pseudocolored by converting to the RGB color palette and setting the green and blue channels to zero (to produce a red image). Note the bright fluorescence intensity exhibited by the filamentous actin network, which extends throughout the cytoplasm.