Club Moss Strobilus Thin Section

FITC-TRITC Bandpass Emission (Dual Band Excitation) Set

Autofluorescence emission intensity from a club moss strobilus (Lycopodium clavatum) thin section. Endogenous autofluorescence in plant tissues arises from a variety of biomolecules, including chlorophyll, carotene, and xanthophyll. In the blue and green excitation regions, chlorophyll has an absorption band with a high extinction coefficient and produces a significant amount of fluorescence when excited with wavelengths between 450 and 550 nanometers. For the club moss tissue illustrated above, note the presence of autofluorescence emission intensity in the green and red spectral regions, which is characteristic of the Nikon FITC-TRITC fluorescence filter combination.