Corn Kernel Thin Section

DAPI-FITC Bandpass Emission (Dual Band Excitation) Set

Autofluorescence emission intensity from a corn kernel (Zea mays) thin section. Endogenous autofluorescence in plant tissues arises from a variety of biomolecules, including chlorophyll, carotene, and xanthophyll. In the ultraviolet and blue excitation regions, chlorophyll has an absorption band with a high extinction coefficient and produces a significant amount of fluorescence when excited with wavelengths between 350 and 500 nanometers. For the corn kernel tissue illustrated above, note the presence of autofluorescence emission intensity in the blue and green spectral regions, which is characteristic of the Nikon DAPI-FITC fluorescence filter combination.