Fern Sporangium

UV-2A Longpass Emission (Wide Bandwidth Excitation) Ultraviolet Set

Autofluorescence emission intensity from a thin section of fern (Cyrtomium falcatum) sporangia. Endogenous autofluorescence in plant tissues arises from a variety of biomolecules, including lignins, chlorophyll, carotene, and xanthophyll. In the ultraviolet region, chlorophyll has an absorption band with a high extinction coefficient and produces a significant amount of fluorescence when excited with wavelengths between 350 and 400 nanometers. Note the presence of spectral bleed-through from autofluorescence emission in the green and red spectral regions, which is strongly reminiscent of a darkfield image.

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