Fern Strobilus Thin Section

CFP Bandpass Emission (Narrow Bandwidth Excitation) Blue-Violet Set

Autofluorescence emission intensity from a thin section of fern strobilus (Cyrtomium falcatum) tissue. Endogenous autofluorescence in plant tissues arises from a variety of biomolecules, including lignins, chlorophyll, carotene, and xanthophyll. In the blue-violet region, chlorophyll has an absorption band with a high extinction coefficient and produces a significant amount of fluorescence when excited with wavelengths between 400 and 440 nanometers. Note the absence of spectral bleed-through from autofluorescence emission in the green and red spectral regions with the CFP filter combination.