HeLa Cell Histones
Cy5 HYQ Bandpass Emission (Wide Bandwidth Excitation) Red Set
Fluorescence emission intensity from a culture of HeLa cells that were immunofluorescently labeled with primary anti-histone (pan) mouse monoclonal antibodies followed by goat anti-mouse Fab fragments conjugated to Alexa Fluor 647. The visible light absorption maximum of Alexa Fluor 647 is 649 nanometers and the emission maximum occurs at 666 nanometers (in the deep red region of the visible light spectrum). The primary antibody, produced from purified nuclear fractions of HeLa cells, binds specifically to an antigen determinant that is present on all five histone proteins (H1, H2A, H2B, H3, and H4). The image was captured in 12-bit grayscale mode, but subsequently pseudocolored by converting to the RGB color palette and setting the green and blue channels to zero (to produce a red image). Note the strong fluorescence intensity from the interphase nuclei.