HeLa Cells with Dil

Y-2E/C Bandpass Emission (Medium Bandwidth Excitation) Yellow Set

Fluorescence emission intensity from a culture of HeLa cells that were labeled with the lipophilic cell tracer carbocyanine dye, Dil, which targets membranes. The visible light absorption maximum of Dil is 549 nanometers and the emission maximum occurs at 565 nanometers (in the yellow region of the visible light spectrum). Fluorescence intensity arising from labeled cytoskeletal membrane components is quite evident in the image even through the probe emission peak is 30 nanometers below the Y-2E/C filter set dichromatic mirror cut-on wavelength.