Human Lung Carcinoma Mitochondria with Alexa Fluor 488
YFP Bandpass Emission (Narrow Bandwidth Excitation) Blue-Green Set
Fluorescence emission intensity from a culture of human lung carcinoma epithelial cells (A-549 line) that were immunofluorescently labeled with primary anti-oxphos complex V inhibitor protein monoclonal antibodies (mouse) followed by goat anti-mouse Fab fragments conjugated to Alexa Fluor 488. The visible light absorption maximum of Alexa Fluor 488 is 495 nanometers and the emission maximum occurs at 519 nanometers, values that fall within the useful range of the Nikon YFP HYQ filter combination. In addition, the specimen was simultaneously stained for DNA with the ultraviolet-absorbing probe DAPI, and for the cytoskeletal filamentous actin network with Alexa Fluor 568 conjugated to phalloidin. Note the high level of green fluorescence from the labeled intracellular mitochondria, but an absence of detected emission from the blue and orange fluorophores (DAPI and Alexa Fluor 568) targeting the nuclei and actin, respectively.