Human Osteosarcoma Cells with DAPI, MitoTracker Red CMXRos, and Alexa Fluor 488
DAPI-FITC-Texas Red Bandpass Emission (Triple Band Excitation) Set
Fluorescence emission intensity from a culture of human osteosarcoma cells (U-2 line) stained with MitoTracker Red CMXRos and Alexa Fluor 488 conjugated to phalloidin, which target the intracellular mitochondrial network and cytoskeletal actin filaments, respectively. The visible light absorption maximum of MitoTracker Red CMXRos is 579 nanometers and the emission maximum occurs at 599 nanometers, while the corresponding values for Alexa Fluor 488 are 495 and 519 nanometers. In addition, the specimen was simultaneously stained with DAPI (targeting DNA in the cell nucleus; ultraviolet excitation with blue emission). Note the presence of high signal levels from all three of the fluorophores employed to stain the specimen.