Human Prostate Gland Tissue

Alexa Fluor 350 (blue fluorescence emission) conjugated to the lectin wheat germ agglutinin, which selectively binds to N-acetylglucosamine and N-acetylneuraminic (sialic acid) residues and is commonly used for staining the Golgi complex, was utilized to label the human prostate gland tissue sample presented above. The specimen was counterstained with Alexa Fluor 488 (green emission) conjugated to phalloidin in order to localize filamentous actin and with SYTOX Orange, a popular nucleic acid stain. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.

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