Indian Muntjac Actin Cytoskeleton

Cy5 HYQ Bandpass Emission (Wide Bandwidth Excitation) Red Set

Fluorescence emission intensity from a culture of Indian Muntjac deerskin fibroblast cells stained with Alexa Fluor 633 conjugated to phalloidin, which targets actin filaments in the cytoskeleton. The visible light absorption maximum of Alexa Fluor 633 is 621 nanometers and the emission maximum occurs at 639 nanometers. The image was captured in 12-bit grayscale mode, but subsequently pseudocolored by converting to the RGB color palette and setting the green and blue channels to zero (to produce a red image). Note the bright fluorescence intensity exhibited by the actin network, which extends throughout the cytoplasm.