Indian Muntjac Cell Actin Cytoskeleton

DAPI-FITC Bandpass Emission (Dual Band Excitation) Set

Fluorescence emission intensity from a culture of Indian Muntjac deerskin fibroblast cells stained with Alexa Fluor 488 conjugated to phalloidin, which binds to the intracellular filamentous actin network. The visible light absorption maximum of Alexa Fluor 488 is 495 nanometers and the emission maximum occurs at 519 nanometers in the green region of the spectrum. In addition, the specimen was simultaneously stained with DAPI (targeting DNA in the cell nucleus; excitation at 358 nanometers and emission at 461 nanometers) and MitoTracker Red CMXRos (targeting mitochondria; red emission). Note the absence of signal from the red fluorophore (MitoTracker), but the presence of bright green fluorescence exhibited by the actin filaments and the slightly lower intensity blue signal from DAPI in the cell nucleus.