Indian Muntjac Cells with LysoTracker

UV-2A Longpass Emission (Wide Bandwidth Excitation) Ultraviolet Set

Fluorescence emission intensity from a culture of Indian Muntjac deerskin fibroblast cells adherent on a cover glass. Nuclei in the cells were targeted with the nucleic acid probe SYTOX Green, which has an excitation maximum at 504 nanometers and an emission maximum at 523 nanometers when bound to DNA in cultured cells and tissue sections. In addition, the cells were stained with LysoTracker Blue DND-22 (targeting lysosomes; excitation at 373 nanometers and emission at 422 nanometers) and Alexa Fluor 568 phalloidin (targeting actin; excitation at 578 nanometers and emission at 600 nanometers). Note the presence of signal from both the red and green fluorophores and reduced contrast when compared to the other Nikon longpass (UV-1A and UV-2B) filter combinations.

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