Indian Muntjac Chromosomes

UV-2E/C Bandpass Emission (Medium Bandwidth Excitation) Ultraviolet Set

Fluorescence emission intensity from a culture of Indian Muntjac deerskin fibroblast cells adherent on a cover glass. Nuclei in the cells were targeted with the nucleic acid probe Hoechst 33258, which has an excitation maximum at 346 nanometers and an emission maximum at 460 nanometers when bound to DNA in cultured cells and tissue sections. In addition, the cells were counterstained with MitoTracker Red CMXRos (mitochondria) and BODIPY FL phallacidin (actin; green fluorescence emission). Note the absence of fluorescence signal from the red and green probes.